WB result of Phospho-CaMKII (Thr286) Recombinant Rabbit mAb
Primary antibody: Phospho-CaMKII (Thr286) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated mouse brain lysate 20 µg
Lane 2: Lambda phosphatase treated mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 48, 60 kDa
Phospho-CaMKII (Thr286) Recombinant Rabbit mAb (S-1251-278)
Phospho-CaMKII (Thr286) Recombinant Rabbit mAb (S-1251-278)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-CaMKII (Thr286) |
| Synonyms | Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha; CAMKA; KIAA0968; CAMK2A |
| Immunogen | Synthetic Peptide |
| Location | Synapse |
| Accession | Q9UQM7 |
| Clone Number | S-1251-278 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Human brain, mouse brain, rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Ms, Rt |
Background
Phospho-CaMKII (Thr286) refers to the phosphorylated form of the Ca2+/calmodulin-dependent protein kinase II (CaMKII) at threonine residue 286. CaMKII is a multifunctional serine/threonine protein kinase that plays a crucial role in various cellular processes, particularly in the heart and brain. When phosphorylated at Thr286, CaMKII undergoes autophosphorylation, which enhances its activity and allows it to function in a Ca2+-independent manner. This phosphorylated state is essential for the regulation of cardiac myocyte contraction, synaptic plasticity, and memory formation. Abnormal phosphorylation of CaMKII at Thr286 has been implicated in several pathological conditions, including heart failure and arrhythmias, making it a potential therapeutic target for cardiovascular diseases.
Picture
Picture
Western Blot
WB result of Phospho-CaMKII (Thr286) Recombinant Rabbit mAb
Primary antibody: Phospho-CaMKII (Thr286) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated rat brain lysate 20 µg
Lane 2: Lambda phosphatase treated rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 48, 60 kDa
Dot Blot
Dot blot result of Phospho-CaMKII (Thr286) Recombinant Rabbit mAb
Lane 1: CaMKII (Thr286) phospho peptide
Lane 2: CaMKII unmodified peptide
Primary antibody: Phospho-CaMKII (Thr286) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
