WB result of Phospho-Bcl-2 (Ser70) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-Bcl-2 (Ser70) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated Jurkat whole cell lysate 20 µg
Lane 2: Jurkat treated with 1 µM paclitaxel for 24 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 26 kDa
Observed MW: 25 kDa
Phospho-Bcl-2 (Ser70) Recombinant Rabbit mAb (S-1344-257)
Phospho-Bcl-2 (Ser70) Recombinant Rabbit mAb (S-1344-257)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-Bcl-2 (Ser70) |
| Synonyms | Apoptosis regulator Bcl-2; BCL2 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Mitochondrion, Endoplasmic reticulum |
| Accession | P10415 |
| Clone Number | S-1344-257 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
Background
Phospho-Bcl-2 (Ser70) is the B-cell lymphoma 2 (Bcl-2) protein bearing a phosphate group on serine-70, a post-translational modification that dynamically tunes its anti-apoptotic function by altering its interaction with pro-apoptotic BH3-only proteins and modulating mitochondrial outer-membrane permeabilization, thereby serving as a key regulatory switch in intrinsic apoptosis signaling and a biomarker for chemoresistance in hematologic and solid tumors.
Picture
Picture
Western Blot
WB result of Phospho-Bcl-2 (Ser70) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-Bcl-2 (Ser70) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat treated with 1 µM paclitaxel for 24 hours whole cell lysate 20 µg
Lane 2: Jurkat treated with 1 µM paclitaxel for 24 hours, then treated with phosphatase whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 26 kDa
Observed MW: 25 kDa
