WB result of Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 25 µg/ml Anisomycin for 35 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52, 54 kDa
Observed MW: 65 kDa
This blot was developed with high sensitivity substrate
Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) Recombinant Rabbit mAb (S-3535)
Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) Recombinant Rabbit mAb (S-3535)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) |
| Synonyms | HB16; ATF2; CREB2; CREBP1; ATFA; ATF7 |
| Location | Cytoplasm, Nucleus |
| Accession | P17544、P15336 |
| Clone Number | S-3535 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Anisomycin treated HeLa |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:250 | Hu, Ms, Rt |
Background
Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) protein is the phosphorylated form of the transcription factors ATF-2 and ATF-7 at threonine 71 and 53, respectively, which enhances their transcriptional activity by promoting dimerization and DNA binding, thereby regulating stress-responsive, inflammatory, and apoptotic gene expression through the JNK and p38 MAPK signaling pathways.
Picture
Picture
Western Blot
WB result of Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 25 µg/ml Anisomycin for 35 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52, 54 kDa
Observed MW: 65 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti- Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti- Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti- Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti- Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti- Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti- Phospho-ATF-2 (Thr71)/ATF-7 (Thr53) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
