WB result of Phospho-AMPKα (Thr172) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-AMPKα (Thr172) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg (phosphatase treated membrane)
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 62 kDa
This blot was developed with high sensitivity substrate
Phospho-AMPKα (Thr172) Recombinant Rabbit mAb (S-2216-10)
Phospho-AMPKα (Thr172) Recombinant Rabbit mAb (S-2216-10)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-AMPKα (Thr172) |
| Synonyms | 5'-AMP-activated protein kinase catalytic subunit alpha; AMPK subunit alpha; Acetyl-CoA carboxylase kinase (ACACA kinase); Hydroxymethylglutaryl-CoA reductase kinase (HMGCR kinase); Tau-protein kinase PRKAA1; PRKAA1; AMPK1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q13131、P54646 |
| Clone Number | S-2216-10 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:500-1:1000 | Hu, Ms, Rt, Mk |
Background
Phospho-AMPKα (Thr172) protein refers to the catalytic α-subunit of AMP-activated protein kinase that has been phosphorylated on threonine 172, a post-translational modification catalyzed primarily by the upstream kinase LKB1 (or CaMKK2 in response to calcium signals) that is essential for switching AMPK from an inactive to an active conformation; once phosphorylated, this evolutionarily conserved serine/threonine kinase acts as a master cellular energy sensor, triggering catabolic pathways such as fatty-acid oxidation and autophagy while simultaneously inhibiting energy-consuming anabolic processes like gluconeogenesis, lipogenesis and protein synthesis, thereby restoring ATP levels during metabolic stress, and its phosphorylation status is widely used as a biomarker for metabolic activity, exercise physiology, mitochondrial health and the efficacy of drugs ranging from metformin to direct AMPK activators in diabetes, cancer and cardiovascular research.
Picture
Picture
Western Blot
WB result of Phospho-AMPKα (Thr172) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-AMPKα (Thr172) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg (phosphatase treated membrane)
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 62 kDa
This blot was developed with high sensitivity substrate
WB result of Phospho-AMPKα (Thr172) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-AMPKα (Thr172) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 starve overnight, then treated with 5 μM Oligomycin for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 62 kDa
This blot was developed with high sensitivity substrate
Dot Blot
Dot blot result of Phospho-AMPKα (Thr172) Recombinant Rabbit mAb
Lane 1: AMPKα (Thr172) phospho peptide
Lane 2: AMPKα (Thr172) unmodified peptide
Primary antibody: Phospho-AMPKα (Thr172) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
