Flow cytometric analysis of Mouse NK1.1/CD161 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Alexa Fluor® 488 Rat Anti-Mouse CD49b antibody and either PerCP-Cy5.5 Rabbit IgG Isotype Control (Left panel) or SDT PerCP-Cy5.5 Rabbit Anti-Mouse NK1.1/CD161 Antibody (Right panel) at 1 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
PerCP-Cy5.5 Rabbit Anti-Mouse NK1.1/CD161 Antibody (S-428-32)
PerCP-Cy5.5 Rabbit Anti-Mouse NK1.1/CD161 Antibody (S-428-32)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | NK1.1/CD161 |
| Synonyms | Killer cell lectin-like receptor subfamily B member 1C; CD161 antigen-like family member C; Lymphocyte antigen 55c (Ly-55c); NKR-P1.9; NKR-P1C; Natural killer cell surface protein P1-40 (NKR-P1 40); CD161c; Klrb1c |
| Immunogen | Recombinant Protein |
| Location | Membrane |
| Accession | P27814 |
| Clone Number | S-428-32 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | PerCP-Cy5.5 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5 μl per million cells in 100μl volume | Ms |
Background
CD161 is a C-type lectin-like membrane receptor expressed at high levels on NK, NKT, Th17, Mucosa Associated Invariant T cells (MAITs), non-MAIT CD8+, tissue-resident memory cells (TRMs), αβ CD4+ T cells and CD8+ T cells. CD161 levels increase when αβ T cells enter tissues. The ligand for CD161 is the lectin-like transcript 1 (LLT1). CD161/LLT1 engagement inhibits NK cell function but can inhibit or enhance αβ T cell function depending on the co-stimulatory molecules involved. The inhibitory capacity of αβ T cells can be neutralized by blocking CD161. CD161 expression on γδ T cells is associated with enhanced IFN-γ and IL-17 production and enhanced endothelial transmigration.
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