Multiparameter flow cytometric analysis of Human CD64 expression on human peripheral blood mononuclear cell populations. Human peripheral blood mononuclear cells were stained with Alexa Fluor® 647 Mouse Anti-Human CD14 antibody and either PE Mouse IgG1, κ Isotype Control (Left panel) or SDT PE Mouse Anti-Human CD64 antibody (Right panel) at 0.1 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
PE Mouse Anti-Human CD64 Antibody (S-R482)
PE Mouse Anti-Human CD64 Antibody (S-R482)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD64 |
| Synonyms | High affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I; Fc-gamma RI (FcRI); Fc-gamma RIA (FcgammaRIa); FCGR1A; FCG1; FCGR1; IGFR1 |
| Location | Cell membrane |
| Accession | P12314 |
| Clone Number | S-R482 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 0.02mg/ml |
| Conjugation | PE |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5 μl per million cells in 100μl volume | Hu |
Background
CD64 is part of the immunoglobulin superfamily and plays a pivotal role in connecting humoral and cellular immunity. The extracellular domain of CD64 contains three immunoglobulin-like domains that readily bind to monomeric IgG. It has a higher affinity for IgG compared to CD32 (FcγRII) and CD16 (FcγRIII), and it shows different binding strengths to various IgG subclasses, with the order of affinity being IgG1/IgG3 > IgG4 > IgG2. CD64 is regulated by cytokines and is involved in the clearance of pathogens through antibody-dependent cellular cytotoxicity, phagocytosis, and immune complex removal. In the context of infectious diseases, CD64 has demonstrated high sensitivity and specificity in diagnosing such conditions compared to other markers like white blood cell count, C-reactive protein (CRP), and procalcitonin (PCT). It can be particularly useful in diagnosing bacterial infections, with sensitivity and specificity rates exceeding 90%. Moreover, the CD64 index has been found to correlate with the severity of sepsis and organ failure, making it a potential early predictor of mortality in sepsis patients.
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