Flow cytometric analysis of Human CD80 expression on Raji. Cells from the Raji (Human Burkitt's lymphoma B lymphocyte, Right) or 293T (Human embryonic kidney epithelial cell, Left) was stained with PE-Cy7 Rabbit IgG Isotype Control (Black line histogram) and SDT PE-Cy7 Rabbit Anti-Human CD80 Antibody (Red line histogram) at 0.1 μg/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
PE-Cy7 Rabbit Anti-Human CD80 Antibody (S-288-177)
PE-Cy7 Rabbit Anti-Human CD80 Antibody (S-288-177)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD80 |
| Synonyms | T-lymphocyte activation antigen CD80, Activation B7-1 antigen, BB1, CTLA-4 counter-receptor B7.1 (B7) |
| Immunogen | Recombinant Protein |
| Location | Membrane |
| Accession | P33681 |
| Clone Number | S-288-177 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.02mg/ml |
| Conjugation | PE-Cy7 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 0.1% BSA, 0.01% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5 μl per million cells in 100μl volume |
Background
The Cluster of differentiation 80 (also CD80 and B7-1) is a B7, type I membrane protein in the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is closely related to CD86, another B7 protein (B7-2), and often works in tandem. Both CD80 and CD86 interact with costimulatory receptors CD28, CTLA-4 (CD152) and the p75 neurotrophin receptor [PMID: 7545666].
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