WB result of PCBP4 Recombinant Rabbit mAb
Primary antibody: PCBP4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HL-60 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Negative control: HL-60 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 41 kDa
This blot was developed with high sensitivity substrate
PCBP4 Recombinant Rabbit mAb (S-3107-175)
PCBP4 Recombinant Rabbit mAb (S-3107-175)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PCBP4 |
| Synonyms | Poly(rC)-binding protein 4 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P57723 |
| Clone Number | S-3107-175 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, NIH/3T3, PC-12, COS-7 |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
Background
PCBP4 (Poly(rC) Binding Protein 4), also known as MCG10 or hnRNP E4, is an RNA-binding protein belonging to the hnRNP K homology domain-containing family. It regulates mRNA stability, translation, and alternative splicing by binding to poly(C) motifs in target transcripts. PCBP4 plays critical roles in cellular stress responses, cell cycle arrest, and apoptosis through post-transcriptional control of genes involved in proliferation and DNA damage response. Its expression is frequently downregulated in various cancers, functioning as a tumor suppressor by stabilizing mRNAs encoding cell cycle inhibitors and pro-apoptotic factors.
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Western Blot
WB result of PCBP4 Recombinant Rabbit mAb
Primary antibody: PCBP4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 41 kDa
This blot was developed with high sensitivity substrate
WB result of PCBP4 Recombinant Rabbit mAb
Primary antibody: PCBP4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 41 kDa
This blot was developed with high sensitivity substrate
WB result of PCBP4 Recombinant Rabbit mAb
Primary antibody: PCBP4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 41 kDa
This blot was developed with high sensitivity substrate
