WB result of PAX5 Rabbit mAb
Primary antibody: PAX5 Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: Ramos whole cell lysate 20 µg
Lane 3: Raji whole cell lysate 20 µg
Lane 4: Daudi whole cell lysate 20 µg
Negative control: Jurkat whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 42 kDa
Exposure time: 180 s
PAX5 Recombinant rabbit mAb (SDT-120-73)
PAX5 Recombinant rabbit mAb (SDT-120-73)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PAX5 |
| Synonyms | Paired box protein Pax-5, B-cell-specific transcription factor, BSAP |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q02548 |
| Clone Number | SDT-120-73 |
| Antibody Type | Rabbit mAb |
| Application | WB, IHC-P, IP |
| Reactivity | Hu, Rt |
| Purification | Protein A |
| Concentration | 0.5mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IHC-P | 1:100 | |
| IP | 1:25 |
Background
The transcription factor Pax5 is essential for commitment of lymphoid progenitors to the B lymphocyte lineage. Pax5 fulfils a dual role by repressing B lineage 'inappropriate' genes and simultaneously activating B lineage–specific genes. This transcriptional reprogramming restricts the broad signaling capacity of uncommitted progenitors to the B cell pathway, regulates cell adhesion and migration, induces VH-DJH recombination, facilitates (pre-)B cell receptor signaling and promotes development to the mature B cell stage. Conditional Pax5 inactivation in early and late B lymphocytes revealed an essential role for Pax5 in controlling the identity and function of B cells throughout B lymphopoiesis. PAX5 has also been implicated in human B cell malignancies, as it is deregulated by chromosomal translocations in a subset of acute lymphoblastic leukemias and non-Hodgkin lymphomas.
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Picture
Western Blot
IP
PAX5 Rabbit mAb at 1/25 dilution (2µg) immunoprecipitating PAX5 in 0.4mg Daudi whole cell lysate.
Western blot was performed on the immunoprecipitate using PAX5 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: Daudi whole cell lysate 10µg (input)
Lane 2: PAX5 Rabbit mAb IP in Daudi whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in Daudi whole cell lysate
Predicted MW: 42 kDa
Observed MW: 42 kDa
Exposure time: 180s
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-PAX5 antibody was used at 1/100 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human Diffuse large B-cell lymphoma (DLBCL).
Anti-PAX5 antibody was used at 1/100 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen.
Anti-PAX5 antibody was used at 1/100 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
