WB result of Park7/DJ-1 Recombinant Rabbit mAb
Primary antibody: Park7/DJ-1 Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: U-2 OS whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: SK-OV-3 whole cell lysate 20 µg
Lane 5: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 22 kDa
Park7/DJ-1 Recombinant Rabbit mAb (S-980-144)
Park7/DJ-1 Recombinant Rabbit mAb (S-980-144)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Park7/DJ-1 |
| Synonyms | Parkinson disease protein 7; Maillard deglycase; Oncogene DJ1; Parkinsonism-associated deglycase; Protein DJ-1; Protein/nucleic acid deglycase DJ-1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Cell membrane |
| Accession | Q99497 |
| Clone Number | S-980-144 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, ICFCM |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | U-2 OS, Jurkat, MCF7, SK-OV-3, HeLa, NIH/3T3, mouse brain, C6, PC-12, rat brain |
| Predicted Reactivity | Ck, Bv, Hm, Dr, AfGrMk |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000 | Hu, Ms, Rt |
| IHC-P | 1:2000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
| ICFCM | 1:500 | Hu |
Background
Park7 (DJ-1) is a small protein of 189 amino acids that is ubiquitously expressed in almost all cells and tissues, including the brain. It exists as a homodimer and is localized to the cytoplasm, mitochondria, and nucleus. DJ-1 belongs to the ThiJ/PfpI protein superfamily and is highly conserved across all biological kingdoms. Mutations in the Park7 gene, including L166P or deletions, are associated with an early-onset form of familial Parkinson's disease (PD). DJ-1 plays remarkable roles in mitochondrial function and antioxidant effects, and its overexpression can protect cells against dopamine- or MPTP-induced neurotoxicity and oxidative stress. DJ-1 is thought to act as a sensor of cellular redox homeostasis and participate in cytoprotective signaling pathways within the cell. Under oxidative stress, a conserved cysteine residue in DJ-1 (Cys106) is oxidized, which is believed to enable the neuroprotective activity of DJ-1. DJ-1 interacts with various proteins, including RACK1 and MAO-B, to regulate cellular signaling and protect against neurotoxicity. The loss of DJ-1 function can lead to the upregulation of MAO-B, contributing to dopamine metabolic dysfunction and neurodegeneration.
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Western Blot
WB result of Park7/DJ-1 Recombinant Rabbit mAb
Primary antibody: Park7/DJ-1 Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 22 kDa
WB result of Park7/DJ-1 Recombinant Rabbit mAb
Primary antibody: Park7/DJ-1 Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 22 kDa
FC
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte) labelling Park7/DJ-1 antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Park7/DJ-1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Park7/DJ-1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Park7/DJ-1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-Park7/DJ-1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Park7/DJ-1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-Park7/DJ-1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in Jurkat cells. Anti- Park7/DJ-1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
