WB result of Pan-Actin Recombinant Rabbit mAb
Primary antibody: Pan-Actin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 42 kDa
Observed MW: 42 kDa
Pan-Actin Recombinant Rabbit mAb (S-1365-34)
Pan-Actin Recombinant Rabbit mAb (S-1365-34)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Actin |
| Immunogen | Synthetic Peptide |
| Accession | P68133、 P60709、 P63261、 P68032、 P62736、 P63267 |
| Clone Number | S-1365-34 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC, ICFCM |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, HepG2, A431, MCF7, NIH/3T3, RAW264.7,C6, PC-12 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
| ICFCM | 1:500 | Hu |
Background
Actin protein is a highly abundant protein in eukaryotic cells and plays a critical role in various cellular functions. Actin exists in two forms, Globular Actin (G-actin) and Filamentous Actin (F-actin). G-actin is the monomeric, soluble form of the protein, while F-actin refers to the polymerized, filamentous structure. Actin filaments form a dynamic cytoskeleton that provides structural support, enables cell motility, and supports muscle contraction. In muscles, actin molecules twist together to form thin filaments, which interdigitate with thick filaments composed of myosin, the most abundant protein in muscle. When a signal for muscle contraction is sent along a nerve to a muscle cell, actin and myosin are activated. Myosin acts as a motor, hydrolyzing adenosine triphosphate (ATP) to release energy, which causes a myosin filament to move along an actin filament, resulting in the sliding of the two filaments past each other. Actin filaments are highly concentrated at the cell periphery, forming a three-dimensional network beneath the plasma membrane. This network, composed of actin filaments and associated actin-binding proteins (known as the cell cortex), determines cell shape and is involved in various cell surface activities, including movement.
Picture
Picture
Western Blot
WB result of Pan-Actin Recombinant Rabbit mAb
Primary antibody: Pan-Actin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 42 kDa
Observed MW: 42 kDa
WB result of Pan-Actin Recombinant Rabbit mAb
Primary antibody: Pan-Actin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 42 kDa
Observed MW: 42 kDa
FC
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelling Actin antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- Actin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
