WB result of p70 S6 Kinase Recombinant Rabbit mAb
Primary antibody: p70 S6 Kinase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HT-29 whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 70 kDa
p70 S6 Kinase Recombinant Rabbit mAb (S-1278-2)
p70 S6 Kinase Recombinant Rabbit mAb (S-1278-2)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | p70 S6 Kinase |
| Synonyms | Ribosomal protein S6 kinase beta-1, S6K-beta-1, S6K1, 70 kDa ribosomal protein S6 kinase 1 (P70S6K1; p70-S6K 1), Ribosomal protein S6 kinase I, Serine/threonine-protein kinase 14A, p70 ribosomal S6 kinase alpha (p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA), RPS6KB1, STK14A |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P23443 |
| Clone Number | S-1278-2 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Bv, Rb |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| ICC | 1:100 |
Background
p70 S6 Kinase, also known as 70 kDa ribosomal protein S6 kinase (p70S6K), is a serine/threonine kinase that plays a crucial role in controlling cell cycle, growth, and survival. It is regulated by the phosphoinositide 3-kinase (PI3K)/mammalian target of rapamycin (mTOR) pathway and is activated in response to various stimuli, including growth factors, mitogens, and nutrients. Once activated, p70S6K phosphorylates the S6 protein of the 40S ribosomal subunit, leading to the selective translation of a specific set of mRNAs that contain oligopyrimidine tracts at their 5' end, known as 5'TOP mRNAs. p70S6K has been implicated in various human diseases, including cancer, diabetes, and obesity, making it a potential therapeutic target for drug development. Its enhanced expression or activation has been correlated with poor prognosis in some cancer types, suggesting it may serve as a biomarker for disease monitoring. Moreover, it is considered a promising target for cancer therapies, as it can promote cancer progression through various mechanisms, such as epithelial-mesenchymal transition, cancer stemness, and drug resistance.
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Western Blot
WB result of p70 S6 Kinase Recombinant Rabbit mAb
Primary antibody: p70 S6 Kinase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 70 kDa
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- p70 S6 Kinase antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- p70 S6 Kinase antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
