WB result of Olfm4 Recombinant Rabbit mAb
Primary antibody: Olfm4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse colon lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Negative control: mouse colon lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 100 kDa
Olfm4 Recombinant Rabbit mAb (S-2748-67)
Olfm4 Recombinant Rabbit mAb (S-2748-67)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Olfm4 |
| Synonyms | Olfactomedin-4; OLM4 |
| Immunogen | Synthetic Peptide |
| Location | Secreted, Mitochondrion |
| Accession | Q3UZZ4 |
| Clone Number | S-2748-67 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Ms, Rt |
| Positive Sample | mouse spleen, rat spleen |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:2000 | Ms, Rt |
Background
Olfm4 (olfactomedin-4) is a secreted glycoprotein highly expressed in intestinal stem cells, bone marrow neutrophils and prostate epithelium that acts as a context-dependent modulator of cell adhesion, innate immunity and apoptosis by binding cadherins and lectins, potentiating Wnt/Notch signaling to promote epithelial regeneration, and interacting with GRIM-19 to suppress mitochondrial apoptosis, while its frequent up-regulation in gastric, colorectal and other cancers fuels tumor growth, metastasis and chemoresistance and makes it a valuable serum biomarker.
Picture
Picture
Western Blot
WB result of Olfm4 Recombinant Rabbit mAb
Primary antibody: Olfm4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat colon lysate 20 µg
Lane 2: rat spleen lysate 20 µg
Negative control: rat colon lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 100 kDa
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse small intestine. Anti-Olfm4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse lung. Anti-Olfm4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-Olfm4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse colon. Anti-Olfm4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat lung. Anti-Olfm4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-Olfm4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat colon. Anti-Olfm4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
