NKX6.1 Recombinant Rabbit mAb (S-260-31)
NKX6.1 Recombinant Rabbit mAb (S-260-31)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | NKX6.1 |
| Synonyms | Homeobox protein Nkx-6.1, Homeobox protein NK-6 homolog A, NKX6-1, NKX6A |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P78426 |
| Clone Number | S-260-31 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IP, IF |
| Reactivity | Hu, Ms |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC | 1:200-1:500 | |
| IF | 1:200 | |
| WB | 1:1000 | |
| IP | 1:50 |
Background
Homeobox protein Nkx-6.1 is a protein that in humans is encoded by the NKX6-1 gene. In the pancreas, NKX6.1 is required for the development of beta cells and is a potent bifunctional transcription regulator that binds to AT-rich sequences within the promoter region of target genes.
Picture
Picture
Western Blot
WB result of NKX6.1 Rabbit mAb
Primary antibody: NKX6.1 Rabbit mAb at 1/1000 dilution
Lane 1: RAW 264.7 whole cell lysate 20 µg
Lane 2: MIN6 whole cell lysate 20 µg
Negative control: RAW 264.7 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 38 kDa
Observed MW: 44, 46 kDa
IP
NKX6.1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating NKX6.1 in 0.4 mg β-TC-6 whole cell lysate.
Western blot was performed on the immunoprecipitate using NKX6.1 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: β-TC-6 whole cell lysate 20 µg (Input)
Lane 2: NKX6.1 Rabbit mAb IP in β-TC-6 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in β-TC-6 whole cell lysate
Predicted MW: 38 kDa
Observed MW: 44,46 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human pancreas. Anti-NKX6.1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse pancreas. Anti-NKX6.1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human pancreas. Anti-NKX6.1 antibody was used at 1/200 dilution (green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
