WB result of NFAT2 Recombinant Mouse mAb
Primary antibody: NFAT2 Recombinant Mouse mAb at 1/1000 dilution
Lane 1: Ramos whole cell lysate 20 µg
Lane 2: Raji whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 101 kDa
Observed MW: 75-115 kDa
This blot was developed with high sensitivity substrate
NFAT2 Recombinant Mouse mAb (S-4705)
NFAT2 Recombinant Mouse mAb (S-4705)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | NFAT2 |
| Synonyms | Nuclear factor of activated T-cells, cytoplasmic 1; NF-ATc1; NFATc1Alternative nameNFAT transcription complex cytosolic component (NF-ATc; NFATc); NFATC; NFATC1 |
| Location | Cytoplasm, Nucleus |
| Accession | O95644 |
| Clone Number | S-4705 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Positive Sample | Ramos, Raji |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Hu |
| IHC-P | 1:1000 | Hu |
| ICC | 1:500 | Hu |
Background
NFAT2 (Nuclear Factor of Activated T-cells 2), also known as NFATc1, is a critical transcription factor belonging to the NFAT family that plays a pivotal role in regulating immune responses, particularly in T-cell activation and differentiation. Under resting conditions, NFAT2 resides in the cytoplasm in a highly phosphorylated state; however, upon T-cell receptor stimulation, intracellular calcium levels rise, activating the phosphatase calcineurin, which dephosphorylates NFAT2 to expose its nuclear localization signal, thereby enabling its translocation into the nucleus. Once inside the nucleus, NFAT2 binds to specific DNA sequences often in cooperation with other transcription factors like AP-1 to induce the expression of key cytokines such as interleukin-2 (IL-2), interferon-gamma, and tumor necrosis factor-alpha, which are essential for clonal expansion and the development of effector T cells. Beyond its canonical function in immunity, NFAT2 is implicated in various physiological and pathological processes including osteoclast differentiation, cardiac valve formation, and cancer progression, making it a significant therapeutic target for immunosuppressive drugs like cyclosporine A and tacrolimus, which exert their effects by inhibiting the calcineurin-mediated activation of this protein.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-NFAT2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-NFAT2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-NFAT2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-NFAT2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-NFAT2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in Ramos cells. Anti-NFAT2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
