WB result of N-Ras (mutated Q61R) Rabbit mAb
Primary antibody: N-Ras (mutated Q61R) Rabbit mAb at 1/5000 dilution
Lane 1: SK-MEL-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 20 kDa
N-Ras (mutated Q61R) Recombinant Rabbit mAb (S-R155)
N-Ras (mutated Q61R) Recombinant Rabbit mAb (S-R155)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | GTPase NRas, Transforming protein N-Ras |
| Location | Cell membrane, Golgi apparatus membrane |
| Accession | P01111 |
| Clone Number | S-R155 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000 | Hu |
| IHC-P | 1:500 | Hu |
Background
All mammalian cells express 3 closely related Ras proteins, termed H-Ras, K-Ras, and N-Ras, that promote oncogenesis when they are mutationally activated at codon 12, 13, or 61. N-Ras mutations were more strongly associated with hematopoietic tumors.
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Western Blot
WB result of N-Ras (mutated Q61R) Rabbit mAb
Primary antibody: N-Ras (mutated Q61R) Rabbit mAb at 1/5000 dilution
Lane 1: 293T transfected with empty vector whole cell lysate 20 µg
Lane 2: 293T transfected with N-Ras-Myc-His fusion protein whole cell lysate 20 µg
Lane 3: 293T transfected with N-Ras (mutated Q61R)-Myc-His fusion protein whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 25 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human melanoma. Anti-N-Ras (mutated Q61R) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
