WB result of Myosin Light Chain 2/MLC-2V Rabbit pAb
Primary antibody: Myosin Light Chain 2/MLC-2V Rabbit pAb at 1/1000 dilution
Lane 1: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 19 kDa
Myosin Light Chain 2/MLC-2V Rabbit Polyclonal Antibody
Myosin Light Chain 2/MLC-2V Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Myosin Light Chain 2/MLC-2V |
| Synonyms | Myosin regulatory light chain 2, ventricular/cardiac muscle isoform; MLC-2; Cardiac myosin light chain 2; Myosin light chain 2; slow skeletal/ventricular muscle isoform (MLC-2s/v); Ventricular myosin light chain 2; MLC2; MYL2 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P10916 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Mouse heart muscle, rat heart muscle, human heart muscle |
| Predicted Reactivity | Bv, Rb |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| IF | 1:500 | Ms, Rt |
Background
Myosin Light Chain 2 (MLC-2V) is a critical sarcomeric protein predominantly found in the ventricular muscle of the heart. It plays a significant role in regulating cardiac muscle contraction through its phosphorylation, which affects myosin cycling kinetics, torsion, and overall function. MLC-2V is part of the myosin complex, a hexameric protein that includes myosin heavy chains and light chains. Its specific isoform in the ventricle, MLC-2V, is essential for maintaining proper cardiac function. Mutations in the MLC-2V gene are associated with severe conditions such as hypertrophic cardiomyopathy and infant-onset myopathy. Additionally, recent studies have highlighted its involvement in the structural integrity of both cardiac and slow-twitch skeletal muscles, further emphasizing its importance in muscle physiology.
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Western Blot
WB result of Myosin Light Chain 2/MLC-2V Rabbit pAb
Primary antibody: Myosin Light Chain 2/MLC-2V Rabbit pAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 19 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human heart muscle. Anti- Myosin Light Chain 2/MLC-2V antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse heart muscle. Anti- Myosin Light Chain 2/MLC-2V antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat heart muscle. Anti- Myosin Light Chain 2/MLC-2V antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse heart muscle. Anti- Myosin Light Chain 2/MLC-2V antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat heart. Anti- Myosin Light Chain 2/MLC-2V antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Expression Atlas
Expression of Myosin Light Chain 2/ MLC-2V in tumor tissue.
Expression of Myosin Light Chain 2/ MLC-2V in human tissue.
Expression of Myosin Light Chain 2/ MLC-2V in mouse and rat tissue.
