WB result of MyD88 Rabbit mAb
Primary antibody: MyD88 Rabbit mAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: Raji whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 35 kDa
MyD88 Recombinant Rabbit mAb (S-788-32)
MyD88 Recombinant Rabbit mAb (S-788-32)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | Myeloid differentiation primary response protein MyD88 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q99836 |
| Clone Number | S-788-32 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IP |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
| IP | 1:50 | null |
Background
MyD88, also known as myeloid differentiation primary response gene 88, is a protein encoded by the MyD88 gene. It belongs to the Toll/IL-1R family and the death domain family, and is essentially a cytosolic soluble protein. The structure of MyD88 protein includes an N-terminal death domain (DD), a middle structural domain, and a C-terminal Toll/interleukin-1 receptor (TIR) domain. The DD region can interact with other proteins possessing DD to form homodimers or heterodimers, which further induce the activation of c-Jun N-terminal kinase (JNK) or nuclear factor-κB (NF-κB). It is also associated with TLR-mediated apoptosis. On the other hand, the TIR domain can bind to other TIR domains, mediating signal transduction. MyD88 plays a crucial role in the immune system, serving as a key adaptor molecule in the Toll-like receptor (TLR) signaling pathway. Primarily located in the cytoplasm, it functions as a pure adapter connecting IL-1R1 to downstream IRAK kinases. By recognizing pathogens, MyD88 induces the production of proinflammatory cytokines and upregulation of costimulatory molecules, triggering rapid activation of the innate immune response. Additionally, MyD88 is involved in regulating genes related to immunity, inflammation, and cell survival.
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Western Blot
WB result of MyD88 Rabbit mAb
Primary antibody: MyD88 Rabbit mAb at 1/1000 dilution
Lane 1: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 33 kDa
WB result of MyD88 Rabbit mAb
Primary antibody: MyD88 Rabbit mAb at 1/1000 dilution
Lane 1: rat spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 33 kDa
IP
MyD88 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating MyD88 in 0.4 mg K562 whole cell lysate.
Western blot was performed on the immunoprecipitate using MyD88 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: K562 whole cell lysate 20 µg (Input)
Lane 2: MyD88 Rabbit mAb IP in K562 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in K562 whole cell lysate
Predicted MW: 33 kDa
Observed MW: 35 kDa
