WB result of Musashi 1/Msi1 Recombinant Rabbit mAb
Primary antibody: Musashi 1/Msi1 Recombinant Rabbit mAb at 1/500 dilution
Lane 1: SH-SY5Y whole cell lysate 40 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 39 kDa
Musashi 1/Msi1 Recombinant Rabbit mAb (S-1477-58)
Musashi 1/Msi1 Recombinant Rabbit mAb (S-1477-58)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Musashi 1/Msi1 |
| Synonyms | RNA-binding protein Musashi homolog 1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | O43347 |
| Clone Number | S-1477-58 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC, ICFCM |
| Reactivity | Hu, Ms |
| Positive Sample | SH-SY5Y, Neuro-2a |
| Predicted Reactivity | Rt |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500 | Hu, Ms |
| ICC | 1:500 | Hu, Ms |
| ICFCM | 1:50 | Hu |
Background
Musashi-1 is an evolutionarily conserved RNA-binding protein that is selectively expressed in neural stem/progenitor cells within the central nervous system (CNS). It plays a crucial role in the self-renewal and differentiation of these cells by regulating various target mRNAs, including those encoding for Numb and p21(CIP-1). As a transcriptional repressor, Musashi-1 can directly modulate the expression of its target proteins, which are involved in critical cellular processes such as cell proliferation, apoptosis, and tumorigenesis. It has been found to be overexpressed in various solid tumors, including neuroglioma, esophageal, gastric, colorectal, and breast cancers. This overexpression suggests that Musashi-1 may contribute to tumor development and maintenance, possibly by sustaining the stem-like properties of cancer cells and promoting their proliferation. Musashi-1 functions by binding to specific mRNAs and regulating their translation. It can act as a translational suppressor of Numb mRNA and synergistically regulate the Notch signaling pathway, which is important for asymmetric cell division in stem cells. This protein's role in maintaining stem cell functions and its involvement in tumor-related signaling pathways make it a significant protein in the context of cancer research and potential therapeutic targets.
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Western Blot
WB result of Musashi 1/Msi1 Recombinant Rabbit mAb
Primary antibody: Musashi 1/Msi1 Recombinant Rabbit mAb at 1/500 dilution
Lane 1: NIH/3T3 whole cell lysate 40 µg
Lane 2: Neuro-2a whole cell lysate 40 µg
Negative control: NIH/3T3 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 39 kDa
FC
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized SH-SY5Y (Human neuroblastoma epithelial cell) labelling Musashi 1 / Msi1 antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Immunocytochemistry
ICC shows positive staining in SH-SY5Y cells. Anti-Musashi 1 / Msi1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
