WB result of MTCO2 Rabbit pAb
Primary antibody: MTCO2 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: THP-1 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Lane 5: Jurkat whole cell lysate 20 µg
Lane 6: COLO 205 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 26 kDa
Observed MW: 18 kDa
MTCO2 Rabbit Polyclonal Antibody
MTCO2 Rabbit Polyclonal Antibody
Price:
Regular price
$91.00 SGD
Regular price
Sale price
$91.00 SGD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | MTCO2 |
| Synonyms | Cytochrome c oxidase subunit 2; Cytochrome c oxidase polypeptide II; MT-CO2; COII; COX2 |
| Immunogen | Synthetic Peptide |
| Accession | P00403 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | HeLa, THP-1, MCF7, K562, Jurkat, COLO 205 |
| Predicted Reactivity | Cz, Or, Gor, Mq, AfGrMk |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:200 | Hu |
Background
MTCO2 is a subunit of cytochrome c oxidase, which is the last enzyme in the mitochondrial electron transport chain. This enzyme complex drives oxidative phosphorylation and is responsible for the reduction of oxygen to water, a process that is essential for cellular energy metabolism. MTCO2 contributes to cytochrome-c oxidase activity and is predicted to be involved in mitochondrial electron transport from cytochrome c to oxygen, as well as in the positive regulation of vasoconstriction. It is a biomarker of Huntington's disease and stomach cancer, highlighting its importance in human health and disease. MTCO2 also serves as a prognostic marker in certain types of cancer, including liver hepatocellular carcinoma and pancreatic adenocarcinoma.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-MTCO2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-MTCO2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-MTCO2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-MTCO2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
