WB result of MRP2 Recombinant Rabbit mAb
Primary antibody: MRP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled HeLa whole cell lysate 20 µg
Lane 2: unboiled A549 whole cell lysate 20 µg
Lane 3: unboiled HepG2 whole cell lysate 20 µg
Lane 4: unboiled A204 whole cell lysate 20 µg
Negative control: unboiled HeLa whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 174 kDa
Observed MW: 250-400 kDa
MRP2 Recombinant Rabbit mAb (S-2316-41)
MRP2 Recombinant Rabbit mAb (S-2316-41)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | MRP2 |
| Synonyms | ATP-binding cassette sub-family C member 2; Canalicular multidrug resistance protein; Canalicular multispecific organic anion transporter 1; Multidrug resistance-associated protein 2; CMOAT; CMOAT1; CMRP; ABCC2 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | Q92887 |
| Clone Number | S-2316-41 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms |
| Positive Sample | A549, HepG2, A204, mouse liver |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:1000 | Hu |
Background
MRP2 (Multidrug Resistance-associated Protein 2), also known as ABCC2, is a 1,545-amino-acid ATP-binding cassette (ABC) transporter localized almost exclusively to the apical (canalicular) membrane of hepatocytes, where it functions as the primary efflux pump for conjugated organic anions including bilirubin glucuronides, glutathione conjugates, and a wide array of xenobiotics such as anticancer drugs, antibiotics, and environmental toxins, thereby playing a central role in hepatic detoxification and limiting the systemic exposure of tissues to these compounds; genetic mutations in the ABCC2 gene lead to Dubin–Johnson syndrome, a benign autosomal recessive disorder characterized by conjugated hyperbilirubinemia and black liver, whereas acquired MRP2 down-regulation contributes to cholestasis and multidrug resistance in cancer chemotherapy, and its expression is transcriptionally regulated by nuclear receptors like PXR, CAR, and FXR that respond to endobiotics and xenobiotics, making MRP2 a critical determinant of pharmacokinetics, drug–drug interactions, and bile formation.
Picture
Picture
Western Blot
WB result of MRP2 Recombinant Rabbit mAb
Primary antibody: MRP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 174 kDa
Observed MW: 250-400 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-MRP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-MRP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human tonsil. Anti-MRP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-MRP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-MRP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
