WB result of MID51 Recombinant Rabbit mAb
Primary antibody: MID51 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: HT-1080 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 51 kDa
This blot was developed with high sensitivity substrate
MID51 Recombinant Rabbit mAb (S-3570-28)
MID51 Recombinant Rabbit mAb (S-3570-28)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | MID51 |
| Synonyms | Mitochondrial dynamics protein MIEF1; Mitochondrial dynamics protein of 51 kDa; Mitochondrial elongation factor 1; Smith-Magenis syndrome chromosomal region candidate gene 7 protein-like (SMCR7-like protein); SMCR7L; MIEF1 |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion |
| Accession | Q9NQG6 |
| Clone Number | S-3570-28 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | 293T, A549, HT-1080, HeLa, NIH/3T3, mouse testis, PC-12, rat testis, COS-7 |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
Background
MID51 (Mitochondrial Dynamics Protein of 51 kDa), also known as MIEF1 (Mitochondrial Elongation Factor 1) or SMCR7L, is a unique outer mitochondrial membrane (OMM) protein that plays a pivotal and somewhat paradoxical role in regulating mitochondrial dynamics by primarily promoting mitochondrial fusion and inhibiting fission. Unlike the canonical fission mediator Drp1 (Dynamin-related protein 1), which MID51 physically recruits to the mitochondrial surface, MID51 acts as a dominant-negative regulator that sequesters Drp1 into inactive spiral structures, thereby preventing Drp1 from executing membrane constriction and division. Structurally, MID51 contains an N-terminal transmembrane domain anchoring it to the OMM and a C-terminal nucleotidyltransferase-like domain that, despite retaining the structural fold, has lost catalytic activity and instead functions as a protein-protein interaction hub essential for Drp1 binding. Beyond its role in maintaining elongated mitochondrial networks crucial for cellular energy homeostasis and stress resistance, MID51 is implicated in various physiological and pathological processes, including cardiomyocyte function, neuronal development, and viral immune evasion (e.g., by hepatitis C virus), where its dysregulation can lead to fragmented mitochondria, impaired bioenergetics, and increased susceptibility to apoptosis.
Picture
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Western Blot
WB result of MID51 Recombinant Rabbit mAb
Primary antibody: MID51 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 51 kDa
This blot was developed with high sensitivity substrate
WB result of MID51 Recombinant Rabbit mAb
Primary antibody: MID51 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 51 kDa
This blot was developed with high sensitivity substrate
WB result of MID51 Recombinant Rabbit mAb
Primary antibody: MID51 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 51 kDa
This blot was developed with high sensitivity substrate
