WB result of MCL1 Recombinant Rabbit mAb
Primary antibody: MCL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: L-929 whole cell lysate 20 µg
Lane 2: A20 whole cell lysate 20 µg
Lane 3: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 37 kDa
MCL1 Recombinant Rabbit mAb (S-2640-208)
MCL1 Recombinant Rabbit mAb (S-2640-208)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | MCL1 |
| Synonyms | Induced myeloid leukemia cell differentiation protein Mcl-1 homolog; Bcl-2-related protein EAT/mcl1; Mcl1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Mitochondrion |
| Accession | P97287 |
| Clone Number | S-2640-208 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Ms, Rt |
| Positive Sample | L-929, A20, C2C12, rat kidney, rat stomach |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
Background
MCL1 (Myeloid cell leukemia 1) is an anti-apoptotic member of the BCL-2 protein family that plays a crucial role in regulating cell survival and death by binding to and neutralizing pro-apoptotic proteins such as BAK and BIM, thereby preventing mitochondrial outer membrane permeabilization and subsequent activation of the intrinsic apoptotic pathway.
Picture
Picture
Western Blot
WB result of MCL1 Recombinant Rabbit mAb
Primary antibody: MCL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat stomach lysate 20 µg
Lane 2: rat kidney lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 37 kDa
This blot was developed with high sensitivity substrate
