WB result of LY6E/SCA-2 Recombinant Rabbit mAb
Primary antibody: LY6E/SCA-2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: T-47D whole cell lysate 20 µg
Lane 2: HT-29 whole cell lysate 20 µg
Lane 3: HCT 116 whole cell lysate 20 µg
Lane 4: SW480 whole cell lysate 20 µg
Lane 5: SW620 whole cell lysate 20 µg
Lane 6: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 13 kDa
Observed MW: 13 kDa
LY6E/SCA-2 Recombinant Rabbit mAb (S-2681-56)
LY6E/SCA-2 Recombinant Rabbit mAb (S-2681-56)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | LY6E/SCA-2 |
| Synonyms | Lymphocyte antigen 6E; Ly-6E; Retinoic acid-induced gene E protein (RIG-E); Stem cell antigen 2 (SCA-2); Thymic shared antigen 1 (TSA-1); 9804; RIGE; SCA2; TSA1 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | Q16553 |
| Clone Number | S-2681-56 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Positive Sample | T-47D, HT-29, HCT 116, SW480, SW620, HEK-293 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:250 | Hu |
| ICC | 1:500 | Hu |
Background
LY6E/SCA-2 is a ~14 kDa glycosylphosphatidylinositol-anchored cell-surface protein of the Ly6/uPAR superfamily that is constitutively expressed in thymus, spleen, placenta and many epithelia and is strongly up-regulated by type I interferons; it functions as a context-dependent modulator of immunity and oncogenesis by tuning T-cell receptor/CD3ζ phosphorylation, enhancing HIV-1 and other viral replication while conversely restricting coronavirus entry via spike-protein interference, and its over-expression in multiple tumors promotes immune escape, metastasis and chemoresistance, making it a candidate prognostic marker and therapeutic target.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human liver. Anti-LY6E/SCA-2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-LY6E/SCA-2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-LY6E/SCA-2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-LY6E/SCA-2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-LY6E/SCA-2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in T-47D cells. Anti-LY6E/SCA-2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
