WB result of Lp-PLA2 Recombinant Rabbit mAb
Primary antibody: Lp-PLA2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated THP-1 whole cell lysate 20 µg
Lane 2: THP-1 treated with 100 nM PMA for 24 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 45 kDa
Lp-PLA2 Recombinant Rabbit mAb (S-3711-113)
Lp-PLA2 Recombinant Rabbit mAb (S-3711-113)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Lp-PLA2 |
| Synonyms | Platelet-activating factor acetylhydrolase; PAF acetylhydrolase; 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; Group-VIIA phospholipase A2 (gVIIA-PLA2); LDL-associated phospholipase A2 (LDL-PLA(2)); PAF 2-acylhydrolase; PAFAH; PLA2G7 |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | Q13093 |
| Clone Number | S-3711-113 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | THP-1 (treated with 100 nM PMA for 24 hours), PC-3, mouse liver, rat liver, rat lung |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
Background
Lipoprotein-associated phospholipase A2 (Lp-PLA2), also known as plasma platelet-activating factor acetylhydrolase, is an enzyme encoded by the PLA2G7 gene and belongs to the phospholipase A2 superfamily. It is primarily associated with lipoproteins in the bloodstream and plays a crucial role in lipid metabolism and inflammation. Lp-PLA2 hydrolyzes oxidized phospholipids, such as oxidized low-density lipoprotein (oxLDL), into lysophosphatidylcholine and non-esterified fatty acids, contributing to the progression of atherosclerosis. Additionally, it acts as a biomarker for vascular inflammation and is linked to various cardiovascular diseases. Recent studies have also revealed its involvement in ferroptosis, a form of regulated cell death, where Lp-PLA2 negatively regulates lipid peroxidation and protects cells from ferroptotic cell death.
Picture
Picture
Western Blot
WB result of Lp-PLA2 Recombinant Rabbit mAb
Primary antibody: Lp-PLA2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 45 kDa
This blot was developed with high sensitivity substrate
WB result of Lp-PLA2 Recombinant Rabbit mAb
Primary antibody: Lp-PLA2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 45 kDa
This blot was developed with high sensitivity substrate
WB result of Lp-PLA2 Recombinant Rabbit mAb
Primary antibody: Lp-PLA2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Lane 2: rat lung lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 45 kDa
