WB result of KLK6 Recombinant Rabbit mAb
Primary antibody: KLK6 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: U-2 OS whole cell lysate 20 µg
Lane 3: TE-1 whole cell lysate 20 µg
Lane 4: HT-29 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Low expression control: U-2 OS whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 27 kDa
KLK6 Recombinant Rabbit mAb (S-2492-111)
KLK6 Recombinant Rabbit mAb (S-2492-111)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | KLK6 |
| Synonyms | Kallikrein-6; Neurosin; Protease M; SP59; Serine protease 18; Serine protease 9; Zyme; PRSS18; PRSS9 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Secreted, Mitochondrion, Endoplasmic reticulum |
| Accession | Q92876 |
| Clone Number | S-2492-111 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Positive Sample | TE-1, HT-29 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
Background
KLK6 (kallikrein-related peptidase 6) is a secreted serine protease encoded on chromosome 19q13.3–13.4 that exerts context-dependent regulation of tumor behavior by cleaving extracellular matrix proteins and activating protease-activated receptors, thereby promoting proliferation, migration, invasion, angiogenesis, and chemoresistance in gastric, colorectal, lung, bladder, and other cancers while also modulating epithelial-to-mesenchymal transition and macrophage–tumor crosstalk via PAR1/PAR2 signaling cascades, making its elevated expression a consistent biomarker of poor prognosis.
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Western Blot
