WB result of IL-8 Recombinnat Rabbit mAb
Primary antibody: IL-8 Rabbit mAb at 1/1000 dilution
Lane 1: untreated PC-3 whole cell lysate 20 µg
Lane 2: PC-3 treated with 2 µg/ml LPS and 500 ng/ml Brefeldin A for 5 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 11 kDa
Observed MW: 12 kDa
IL-8 Recombinant Rabbit mAb (S-162-38)
IL-8 Recombinant Rabbit mAb (S-162-38)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | IL-8 |
| Synonyms | Interleukin-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1 (GCP-1), Monocyte-derived neutrophil chemotactic factor (MDNCF), Monocyte-derived neutrophil-activating peptide (MONAP), Neutrophil-activating protein 1 (NAP-1), Protein 3-10C, T-cell chemotactic factor, CXCL8 |
| Immunogen | Recombinant Protein |
| Location | Secreted |
| Accession | P10145 |
| Clone Number | S-162-38 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC, IP |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
| IP | 1:50 | null |
| ICC | 1:500 | null |
Background
Interleukin 8 (IL-8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells and endothelial cells. Endothelial cells store IL-8 in their storage vesicles, the Weibel-Palade bodies. IL-8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also stimulates phagocytosis once they have arrived. IL-8 is also known to be a potent promoter of angiogenesis. In target cells, IL-8 induces a series of physiological responses required for migration and phagocytosis, such as increases in intracellular Ca2+, exocytosis (e.g. histamine release), and the respiratory burst. Interleukin-8 is a key mediator associated with inflammation where it plays a key role in neutrophil recruitment and neutrophil degranulation. As an example, it has been cited as a proinflammatory mediator in gingivitis and psoriasis.
Picture
Picture
Western Blot
IP
IL-8 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating IL-8 in 0.4 mg PC-3 treated with 2 µg/ml LPS and 500 ng/ml Brefeldin A for 5 hours whole cell lysate.
Western blot was performed on the immunoprecipitate using IL-8 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: PC-3 treated with 2 µg/ml LPS and 500 ng/ml Brefeldin A for 5 hours whole cell lysate 20 µg (Input)
Lane 2: IL-8 Rabbit mAb IP in PC-3 treated with 2 µg/ml LPS and 500 ng/ml Brefeldin A for 5 hours whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in PC-3 treated with 2 µg/ml LPS and 500 ng/ml Brefeldin A for 5 hours whole cell lysate
Predicted MW: 11 kDa
Observed MW: 12 kDa
This blot was developed with high sensitivity substrate
Immunocytochemistry
ICC analysis of PC-3 cells treated with LPS (2μg/ml, 5hr) + Brefeldin A (500ng/ml, 5hr) (top panel) and untreated PC-3 cells (below panel). Anti- IL-8 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
