IHC shows positive staining in paraffin-embedded human lung. Anti- IL-33 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IL-33 Recombinant Rabbit mAb (S-1700-223)
IL-33 Recombinant Rabbit mAb (S-1700-223)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | IL-33 |
| Synonyms | Interleukin-33; Interleukin-1 family member 11 (IL-1F11); Nuclear factor from high endothelial venules (NF-HEV); C9orf26; IL1F11; NFHEV; IL33 |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Nucleus, Secreted |
| Accession | O95760 |
| Clone Number | S-1700-223 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:1000 | Hu |
Background
IL-33 is a dual-function cytokine of the IL-1 family that, after release from damaged or necrotic endothelial, epithelial and fibroblast-like cells, binds its receptor ST2 to drive type-2 immunity by activating NF-κB and MAPK pathways in target cells, thereby promoting the production of IL-4, IL-5 and IL-13, orchestrating allergic inflammation, tissue repair and fibrosis, and serving as a nuclear alarmin that can also translocate to the nucleus to regulate gene expression.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human stomach. Anti- IL-33 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti- IL-33 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti- IL-33 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
