WB result of IL-2 Recombinant Rabbit mAb
Primary antibody: IL-2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated Jurkat whole cell lysate 20 µg
Lane 2: Jurkat treated with 40 nM Phorbol-12-myristate-13-acetate, 2 µM A23187, and 300 ng/ml Brefeldin A (45 min delay) overnight whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 15 kDa
IL-2 Recombinant Rabbit mAb (S-146-223)
IL-2 Recombinant Rabbit mAb (S-146-223)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | IL-2 |
| Synonyms | Interleukin-2, T-cell growth factor (TCGF) |
| Immunogen | Recombinant Protein |
| Location | Secreted |
| Accession | P60568 |
| Clone Number | S-146-223 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
Background
Interleukin 2 (IL-2) is a cytokine that produced predominantly by Th cells, stimulates the growth and differentiation of B cells, T cells, NK cells, and monocyte/macrophages. IL-2 signals through a receptor complex consisting of the IL-2Rα subunit, IL-2Rβ subunit, and γc, which activates the JAK/STAT pathway, especially STAT3 and 5, leading to gene transcription.
Picture
Picture
Western Blot
