WB result of IKK alpha/beta Recombinant Rabbit mAb
Primary antibody: IKK alpha/beta Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HCT 116 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 87 kDa
Observed MW: 85, 90 kDa
IKK alpha/beta Recombinant Rabbit mAb (S-1317-27)
IKK alpha/beta Recombinant Rabbit mAb (S-1317-27)
Price:
Regular price
$130.00 SGD
Regular price
Sale price
$130.00 SGD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | IKK alpha/beta |
| Synonyms | Inhibitor of nuclear factor kappa-B kinase subunit alpha, I-kappa-B kinase alpha, IKK-A, IkBKA, IkappaB kinase, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase 1, Nuclear factor NF-kappa-B inhibitor kinase alpha (NFKBIKA), Transcription factor 16 (TCF-16), CHUK, Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B-kinase beta, IKK-B, IkBKB, I-kappa-B kinase 2, Nuclear factor NF-kappa-B inhibitor kinase beta (NFKBIKB), Serine/threonine protein kinase IKBKB, IKBKB |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Nucleus |
| Accession | O14920、 O15111 |
| Clone Number | S-1317-27 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC, ICFCM, IP |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
| IP | 1:50 | null |
| ICC | 1:50 | null |
| ICFCM | 1:50 | null |
Background
The Inhibitor of Nuclear Factor Kappa-B Kinase (IKK) is a pivotal serine/threonine kinase complex that plays a crucial role in regulating the Nuclear Factor Kappa-B (NF-κB) signaling pathway. This pathway is fundamental for controlling diverse physiological and pathological processes, including immune responses, inflammation, cell proliferation, and apoptosis. The IKK complex comprises three main subunits: IKKα (also known as IKK1), IKKβ (IKK2), and IKKγ (or NEMO/IKBKG). Each subunit contributes uniquely to the complex's structure and function. IKKα possesses kinase activity, albeit lower than IKKβ. Its precise role in NF-κB activation remains somewhat elusive, but it may be involved in specific types of NF-κB activation. IKKβ serves as the primary kinase subunit within the complex, exhibiting robust kinase activity. IKKβ is essential for activating the NF-κB pathway by phosphorylating the inhibitory protein IκB, leading to its degradation and subsequent release of NF-κB for nuclear translocation and transcriptional activation. IKK's primary function lies in the activation of the NF-κB signaling pathway. By phosphorylating specific serine residues on the IκB proteins, IKK triggers their ubiquitination and subsequent degradation by the proteasome. This process frees NF-κB from inhibition, allowing it to translocate to the nucleus and activate the transcription of target genes.
Picture
Picture
Western Blot
WB result of IKK alpha/beta Recombinant Rabbit mAb
Primary antibody: IKK alpha/beta Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 87 kDa
Observed MW: 85, 90 kDa
WB result of IKK alpha/beta Recombinant Rabbit mAb
Primary antibody: IKK alpha/beta Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 87 kDa
Observed MW: 85, 90 kDa
FC
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling IKK alpha/beta antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
IP
IKK alpha/beta Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating IKK alpha/beta in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using IKK alpha/beta Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 20 µg (Input)
Lane 2: IKK alpha/beta Rabbit mAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 87 kDa
Observed MW: 85, 90 kDa
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- IKK alpha beta antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
