WB result of Huntingtin Recombinant Rabbit mAb
Primary antibody: Huntingtin gamma Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 348 kDa
Observed MW: 348 kDa
This blot was developed with high sensitivity substrate
Huntingtin Recombinant Rabbit mAb (S-1481-147)
Huntingtin Recombinant Rabbit mAb (S-1481-147)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Huntingtin |
| Synonyms | Huntington disease protein (HD protein); HD; IT15; HTT |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P42858 |
| Clone Number | S-1481-147 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, SH-SY5Y, Neuro-2a, mouse brain, PC-12, rat brain |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| ICC | 1:2000 | Hu |
Background
Huntingtin (HTT) is a large soluble protein composed of approximately 3144 amino acids, with a molecular weight of around 348 kDa. It is highly expressed in the central nervous system, particularly in neurons of the cerebral cortex and hippocampus. Huntingtin plays crucial roles in various cellular processes, including clathrin-mediated endocytosis, axonal transport, and synaptic signal transduction. It also helps protect neurons from apoptosis.
Picture
Picture
Western Blot
WB result of Huntingtin Recombinant Rabbit mAb
Primary antibody: Huntingtin gamma Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 348 kDa
Observed MW: 348 kDa
This blot was developed with high sensitivity substrate
WB result of Huntingtin Recombinant Rabbit mAb
Primary antibody: Huntingtin gamma Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 348 kDa
Observed MW: 348 kDa
This blot was developed with high sensitivity substrate
Immunocytochemistry
ICC shows positive staining in SH-SY5Y cells. Anti- Huntingtin antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
