Human IgG3 F(c) Recombinant Rabbit mAb (HRP Conjugate)
Primary antibody: Human IgG3 F(c) Recombinant Rabbit mAb (HRP Conjugate) at 1/1000 dilution
Lane 1: IgG1 F(c) recombinant protein lysate 10 ng
Lane 2: IgG2 F(c) recombinant protein lysate 10 ng
Lane 3: IgG3 F(c) recombinant protein lysate 10 ng
Lane 4: IgG4 F(c) recombinant protein lysate 10 ng
Predicted MW: 33 kDa
Observed MW: 38 kDa
Product Details
Product Details
Product Specification
Host | Rabbit |
Antigen | Human IgG3 F(c) |
Synonyms | Immunoglobulin heavy constant gamma 3, HDC, Heavy chain disease protein, Ig gamma-3 chain C region, IGHG3 |
Immunogen | Native protein |
Location | Secreted, Cell membrane |
Accession | P01860 |
Clone Number | S-318-77 |
Antibody Type | Recombinant mAb |
Isotype | IgG |
Application | ELISA, WB |
Reactivity | Hu |
Purification | Protein A |
Concentration | 2 mg/ml |
Conjugation | HRP |
Physical Appearance | Liquid |
Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
application | dilution | species |
WB | 1:1000 | null |
Background
The intact antibody of human immunoglobulin (IgG) is composed of the fragment for antigen binding (Fab) and the crystallizable fragment (Fc) for binding of Fcγ receptors. Among the four subclasses of human IgG (IgG1, IgG2, IgG3, IgG4), which differ in their constant regions, particularly in their hinges and CH2 domains, IgG1 has the highest FcγR-binding affinity, followed by IgG3, IgG2, and IgG4.
Picture
Picture
Western Blot
