WB result of Human IgG1 F(c) Rabbit mAb
Primary antibody: Human IgG1 F(c) Rabbit mAb at 1/10000 dilution
Lane 1: Human serum lysate 5 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 52 kDa
Human IgG1 F(c) Recombinant Rabbit mAb (S-447-20)
Human IgG1 F(c) Recombinant Rabbit mAb (S-447-20)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Human IgG1 F(c) |
| Synonyms | Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1 |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | P01857 |
| Clone Number | S-447-20 |
| Antibody Type | Recombinant mAb |
| Application | ELISA, WB, IHC-P, IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.25 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:10000 | |
| IHC | 1:1000 | |
| IF | 1:1000 |
Background
The intact antibody of human immunoglobulin (IgG) is composed of the fragment for antigen binding (Fab) and the crystallizable fragment (Fc) for binding of Fcγ receptors. Among the four subclasses of human IgG (IgG1, IgG2, IgG3, IgG4), which differ in their constant regions, particularly in their hinges and CH2 domains, IgG1 has the highest FcγR-binding affinity, followed by IgG3, IgG2, and IgG4 [PMID: 32370812].
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Western Blot
WB result of Human IgG1 F(c) Rabbit mAb
Primary antibody: Human IgG1 F(c) Rabbit mAb at 1/10000 dilution
Lane 1: Human IgG recombinant protein lysate 20 ng
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 55 kDa
WB result of Human IgG1 F(c) Rabbit mAb
Primary antibody: Human IgG1 F(c) Rabbit mAb at 1/1000 dilution
Lane 1: IgG2 F(c) recombinant protein lysate 10 ng
Lane 2: IgG3 F(c) recombinant protein lysate 10 ng
Lane 3: IgG4 F(c) recombinant protein lysate 10 ng
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: IgG2 F(c)、IgG4 F(c): 27kDa; IgG3 F(c): 33kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Human IgG1 F(c) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-Human IgG1 F(c) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human tonsil. Anti-Human IgG1 F(c) antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
