WB result of Human IgG1 F(c) Mouse mAb (HRP Conjugate)
Primary antibody: Human IgG1 F(c) Mouse mAb (HRP Conjugate) at 1/1000 dilution
Lane 1: human serum lysate 5 µg
Predicted MW: 27 kDa
Observed MW: 55 kDa
Human IgG1 F(c) Mouse mAb (HRP Conjugate) (S-448-1)
Human IgG1 F(c) Mouse mAb (HRP Conjugate) (S-448-1)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Human IgG1 F(c) |
| Synonyms | Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1 |
| Immunogen | Synthetic Peptide |
| Location | Secreted, Cell membrane |
| Accession | P01857 |
| Clone Number | S-448-1 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1 |
| Application | ELISA, WB |
| Reactivity | Hu |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | HRP |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
Background
The intact antibody of human immunoglobulin (IgG) is composed of the fragment for antigen binding (Fab) and the crystallizable fragment (Fc) for binding of Fcγ receptors. Among the four subclasses of human IgG (IgG1, IgG2, IgG3, IgG4), which differ in their constant regions, particularly in their hinges and CH2 domains, IgG1 has the highest FcγR-binding affinity, followed by IgG3, IgG2, and IgG4.
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Picture
Western Blot
