WB result of Human IgG F(c) Rabbit mAb
Primary antibody: Human IgG F(c) Rabbit mAb at 1/1000 dilution
Lane 1: human seurm lysate 1 µg
Lane 2: mouse seurm lysate 5 µg
Lane 3: rat seurm lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 55 kDa
Human IgG F(c) Recombinant Rabbit mAb (S-318-184)
Human IgG F(c) Recombinant Rabbit mAb (S-318-184)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Human IgG |
| Synonyms | Immunoglobulin heavy constant gamma |
| Immunogen | Native protein |
| Location | Secreted, Cell membrane |
| Accession | P01859、 P01860、 P01861、 P01857 |
| Clone Number | S-318-184 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ELISA, WB, IHC-P, IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
| IHC-P | 1:4000 | null |
| IF | 1:4000 | null |
Background
The intact antibody of human immunoglobulin (IgG) is composed of the fragment for antigen binding (Fab) and the crystallizable fragment (Fc) for binding of Fcγ receptors. Among the four subclasses of human IgG (IgG1, IgG2, IgG3, IgG4), which differ in their constant regions, particularly in their hinges and CH2 domains, IgG1 has the highest FcγR-binding affinity, followed by IgG3, IgG2, and IgG4 [PMID: 32370812IF: 29.5 Q1 ].
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Human IgG F(c) antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded tonsil. Anti-Human IgG F(c) antibody was used at 1/4000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
