IHC shows positive staining in paraffin-embedded human colon. Anti-Human IgA1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Human IgA1 Rabbit Polyclonal Antibody
Human IgA1 Rabbit Polyclonal Antibody
Price:
Regular price
$110 USD
Regular price
Sale price
$110 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Human IgA1 |
| Synonyms | Immunoglobulin heavy constant alpha 1; Ig alpha-1 chain C region; Ig alpha-1 chain C region BUR; Ig alpha-1 chain C region TRO; IGHA1 |
| Immunogen | Synthetic Peptide |
| Location | Secreted, Cell membrane |
| Accession | P01876-1 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | IHC-P |
| Reactivity | Hu |
| Purification | Immunogen Affinity |
| Concentration | 0,5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:1000-1:2000 | Hu |
Background
Human IgA1 (Immunoglobulin A1) is a type of antibody that plays a crucial role in the immune system. It is the most abundant immunoglobulin found in mucosal secretions such as saliva, tears, and gastrointestinal fluids, where it helps to prevent infections by neutralizing pathogens and preventing their attachment to mucosal surfaces. Structurally, IgA1 consists of two heavy chains and two light chains, with a unique hinge region that makes it more susceptible to proteolytic cleavage compared to other immunoglobulins. This protein is synthesized by plasma cells and is often found in both monomeric and dimeric forms, with the dimeric form being particularly important in mucosal immunity due to its higher avidity. Abnormalities in IgA1, such as glycosylation defects, have been implicated in certain autoimmune and inflammatory diseases, including IgA nephropathy.
Picture
Picture
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human spleen. Anti-Human IgA1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Human IgA1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human appendix. Anti-Human IgA1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-Human IgA1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-Human IgA1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human renal clear cell carcinoma. Anti-Human IgA1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-Human IgA1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
