WB result of HSC70 Recombinant Rabbit mAb
Primary antibody: HSC70 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: MCF-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 71 kDa
Observed MW: 70 kDa
HSC70 Recombinant Rabbit mAb (S-996-65)
HSC70 Recombinant Rabbit mAb (S-996-65)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | HSC70 |
| Synonyms | Heat shock cognate 71 kDa protein; Heat shock 70 kDa protein 8; Heat shock protein family A member 8; Lipopolysaccharide-associated protein 1 (LAP-1, LPS-associated protein 1); HSPA8; HSP73; HSPA10 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane, Cytoplasm, Nucleus |
| Accession | P11142 |
| Clone Number | S-996-65 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC, ICFCM |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Bv, Av, Hr, Or, Hm, Rb |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| ICC | 1:500 | |
| ICFCM | 1:500 |
Background
Heat shock 70 kDa protein 8 also known as heat shock cognate 71 kDa protein or Hsc70 or Hsp73 is a heat shock protein that in humans is encoded by the HSPA8 gene. As a member of the heat shock protein 70 family and a chaperone protein, it facilitates the proper folding of newly translated and misfolded proteins, as well as stabilize or degrade mutant proteins. Its functions contribute to biological processes including signal transduction, apoptosis, autophagy, protein homeostasis, and cell growth and differentiation. It has been associated with an extensive number of cancers, neurodegenerative diseases, cell senescence, and aging.
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Picture
Western Blot
WB result of HSC70 Recombinant Rabbit mAb
Primary antibody: HSC70 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 71 kDa
Observed MW: 70 kDa
WB result of HSC70 Recombinant Rabbit mAb
Primary antibody: HSC70 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 71 kDa
Observed MW: 70 kDa
FC
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) labelling HSC70 antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Immunocytochemistry
ICC shows positive staining in MCF-7 cells. Anti-HSC70 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
