WB result of Histone H3 (tri methyl K27) Recombinant Rabbit mAb
Primary antibody: Histone H3 (tri methyl K27) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
This blot was developed with high sensitivity substrate
Histone H3 (tri methyl K27) Recombinant Rabbit mAb (S-4331)
Histone H3 (tri methyl K27) Recombinant Rabbit mAb (S-4331)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Histone H3 (tri methyl K27) |
| Synonyms | H3K27me3 |
| Location | Nucleus |
| Accession | P68431 |
| Clone Number | S-4331 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, Jurkat, NIH/3T3, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Hu, Ms, Mk |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| ICC | 1:1000 | Hu, Ms |
Background
Histone H3 (tri methyl K27) (H3K27me3) is a crucial epigenetic modification catalyzed by the Polycomb Repressive Complex 2 (PRC2). It functions primarily in the transcriptional repression of target genes and plays a key role in biological processes such as embryonic development, cell fate determination, and genomic imprinting. It is particularly vital for maintaining stem cell pluripotency and establishing cell type-specific gene expression patterns. Aberrant distribution of H3K27me3 is associated with various diseases, including developmental disorders and cancers (such as certain sarcomas and lymphomas), where its excessive silencing can lead to the inactivation of tumor suppressor genes. Common techniques for detecting H3K27me3 include ChIP-seq and immunofluorescence. Furthermore, it is a significant target for current epigenetic drug development, such as EZH2 inhibitors.
Picture
Picture
Western Blot
WB result of Histone H3 (tri methyl K27) Recombinant Rabbit mAb
Primary antibody: Histone H3 (tri methyl K27) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
This blot was developed with high sensitivity substrate
WB result of Histone H3 (tri methyl K27) Recombinant Rabbit mAb
Primary antibody: Histone H3 (tri methyl K27) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-Histone H3 (tri methyl K27) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Histone H3 (tri methyl K27) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-Histone H3 (tri methyl K27) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-Histone H3 (tri methyl K27) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-Histone H3 (tri methyl K27) antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- Histone H3 (tri methyl K27) antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
