WB result of Histone H3 (Lactyl K18) Recombinant Rabbit mAb
Primary antibody: Histone H3 (Lactyl K18) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 mM Lactate sodium for 24 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
Histone H3 (Lactyl K18) Recombinant Rabbit mAb (S-3217-72)
Histone H3 (Lactyl K18) Recombinant Rabbit mAb (S-3217-72)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Histone H3 (Lactyl K18) |
| Synonyms | H3K18la |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P68431 |
| Clone Number | S-3217-72 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa treated with 100 mM Lactate sodium for 24 hours, NIH/3T3 treated with 100 mM Lactate sodium for 24 hours |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000-1:2000 | Hu, Ms |
| ICC | 1:500 | Hu, Ms |
Background
Histone H3 (Lactyl K18) refers to a specific post-translational modification on the 18th lysine residue (K18) of the histone H3 protein, where a lactate-derived lactyl group is covalently attached. Discovered as part of the broader "histone lactylation" mechanism, this epigenetic mark serves as a direct metabolic sensor that links cellular glycolysis and lactate production to gene regulation. Unlike traditional acetylation, which is often associated with general transcriptional activation, H3K18 lactylation (H3K18la) is particularly enriched during specific physiological states such as the late phase of macrophage polarization (driving the transition from pro-inflammatory M1 to reparative M2 phenotypes), hypoxia, and intense exercise. By recruiting specific reader proteins or altering chromatin accessibility at promoter and enhancer regions, H3K18la facilitates the expression of genes involved in tissue repair, angiogenesis, and metabolic adaptation, thereby establishing a crucial mechanistic bridge between cellular metabolism (the Warburg effect or high glycolytic flux) and the epigenetic control of cell fate and function.
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Western Blot
WB result of Histone H3 (Lactyl K18) Recombinant Rabbit mAb
Primary antibody: Histone H3 (Lactyl K18) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 100 mM Lactate sodium for 24 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
Dot Blot
Dot blot result of Histone H3 (Lactyl K18) Recombinant Rabbit mAb
Lane 1: Histone H3K18 lactoylation peptide
Lane 2: Histone H3 unmodified peptide
Primary antibody: Histone H3 (Lactyl K18) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Immunocytochemistry
ICC analysis of HeLa cells treated with Lactate sodium (100 mM, 24 hours) (top panel) and untreated HeLa cells (below panel). Anti-Histone H3 (Lactyl K18) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC analysis of NIH/3T3 cells treated with Lactate sodium (100 mM, 24 hours) (top panel) and untreated NIH/3T3 cells (below panel). Anti-Histone H3 (Lactyl K18) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
