WB result of Histone H3 (citrulline R2 + R8 + R17) Recombinant Rabbit mAb
Primary antibody: Histone H3 (citrulline R2 + R8 + R17) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HEK-293 whole cell lysate 20 µg
Lane 2: HEK-293 transfected with PADI4 expression vector, then treated with 10 mM CaCl2 and 10 µM Ionomycin for 2 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
Histone H3 (citrulline R2 + R8 + R17) Recombinant Rabbit mAb (S-1733-17)
Histone H3 (citrulline R2 + R8 + R17) Recombinant Rabbit mAb (S-1733-17)
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$130.00 SGD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Histone H3 (citrulline R2 + R8 + R17) |
| Synonyms | Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P68431 |
| Clone Number | S-1733-17 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu |
Background
Histone H3 is a core component of the nucleosome, the fundamental unit of chromatin, which plays a crucial role in organizing genomic DNA within the eukaryotic nucleus. The citrullination of Histone H3 at arginine residues R2, R8, and R17 is a specific post-translational modification catalyzed by peptidyl arginine deiminase 4 (PAD4). This modification prevents methylation of these residues by protein-arginine methyltransferase 4 (PRMT4) and is associated with transcriptional repression. Citrullinated Histone H3 is also a key component of neutrophil extracellular traps (NETs) and acts as an autoantigen, contributing to the production of anti-citrullinated protein antibodies in various diseases, including sepsis, multiple sclerosis, rheumatoid arthritis, and multiple myeloma.
Picture
Picture
Western Blot
Dot Blot
Dot blot result of Histone H3 (citrulline R2 + R8 + R17) Recombinant Rabbit mAb
Lane 1: Histone H3 (citrulline R2+R8+R17) modified peptide
Lane 2: Histone H3 (citrulline R2) modified peptide
Lane 3: Histone H3 (citrulline R8) modified peptide
Lane 4: Histone H3 (citrulline R17) modified peptide
Lane 5: Histone H3 (citrulline R2+R8+R17) unmodified peptide
Primary antibody: Histone H3 (citrulline R2 + R8 + R17) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
