WB result of Histone H3 (acetyl K23) Recombinant Rabbit mAb
Primary antibody: Histone H3 (acetyl K23) Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: untreated MCF7 whole cell lysate 20 µg
Lane 2: MCF7 treated with 50 mM sodium butyrate for 24 hours and 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
Histone H3 (acetyl K23) Recombinant Rabbit mAb (S-3321-202)
Histone H3 (acetyl K23) Recombinant Rabbit mAb (S-3321-202)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Histone H3 (acetyl K23) |
| Synonyms | H3K23ac |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P68431 |
| Clone Number | S-3321-202 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | MCF7 (treated with 50 mM sodium butyrate for 24 hours and 500 ng/ml TSA for 4 hours), NIH/3T3 (treated with 500 ng/ml TSA for 4 hours), C6 (treated with 500 ng/ml TSA for 4 hours), HeLa (treated with 500ng/ml TSA for 4 hours) |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt |
| IHC-P | 1:2500 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
Histone H3 lysine 23 acetylation (H3K23ac) is a crucial epigenetic modification characterized by the addition of an acetyl group to the lysine residue at position 23 on the N-terminal tail of histone H3, a process catalyzed by specific histone acetyltransferases (such as p300/CBP) and reversible by deacetylases. Distinct from many other acetylation marks (e.g., H3K9ac, H3K27ac) that primarily enrich at active promoters or enhancers, H3K23ac exhibits a unique genomic distribution; it is widely present in euchromatin and notably enriched within gene bodies, particularly in exons. This specific localization suggests its function extends beyond transcriptional initiation to include roles in regulating transcription elongation, facilitating splice site recognition, and maintaining an open chromatin structure to prevent the erroneous spread of heterochromatin. Furthermore, H3K23ac plays a pivotal role in DNA damage response and specific developmental stages, while its dysregulation has been linked to various cancers and neurodevelopmental disorders, establishing it as an emerging and critical regulatory marker in contemporary epigenetic research.
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Western Blot
WB result of Histone H3 (acetyl K23) Recombinant Rabbit mAb
Primary antibody: Histone H3 (acetyl K23) Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
WB result of Histone H3 (acetyl K23) Recombinant Rabbit mAb
Primary antibody: Histone H3 (acetyl K23) Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
Dot Blot
Dot blot result of Histone H3 (acetyl K23) Recombinant Rabbit mAb
Lane 1: Histone H3 K23ac modified peptide
Lane 2: Histone H3 K23 unmodified peptide
Primary antibody: Histone H3 (acetyl K23) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-Histone H3 (acetyl K23) antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Histone H3 (acetyl K23) antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-Histone H3 (acetyl K23) antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-Histone H3 (acetyl K23) antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat lung. Anti-Histone H3 (acetyl K23) antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC analysis of HeLa cells treated with TSA (500ng/ml, 4h) (top panel) and untreated HeLa cells (below panel). Anti-Histone H3 (acetyl K23) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
