WB result of Histone H2B (mono methyl K5) Recombinant Rabbit mAb
Primary antibody: Histone H2B (mono methyl K5) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: LNCaP whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 14 kDa
Observed MW: 17 kDa
This blot was developed with high sensitivity substrate
Histone H2B (mono methyl K5) Recombinant Rabbit mAb (S-1416-412)
Histone H2B (mono methyl K5) Recombinant Rabbit mAb (S-1416-412)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Histone H2B (mono methyl K5) |
| Synonyms | Histone H2B type 2-E; H2B-clustered histone 21; Histone H2B-GL105; Histone H2B.q (H2B/q); H2BC21; H2BFQ; HIST2H2BE |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q16778 |
| Clone Number | S-1416-412 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, MCF7, LNCaP, NIH/3T3, C6 |
| Predicted Reactivity | Zf, Ck, Or, Cw, X. laevis, M. fascicularis, C. moschata, C. niloticus |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms |
Background
Histone H2B (mono methyl K5) is a specific modification of the histone protein H2B at lysine residue 5, where a single methyl group is added. Histone H2B (mono methyl K5) is a core component of the nucleosome, which is essential for the packaging of DNA into chromatin. This modification, along with others, contributes to the regulation of DNA accessibility, thereby influencing transcription regulation, DNA repair, DNA replication, and chromosomal stability. Histone H2B mono methylation, particularly at K5, has been implicated in transcriptional regulation. It is part of a complex set of post-translational modifications known as the "histone code," which affects gene expression.
Picture
Picture
Western Blot
WB result of Histone H2B (mono methyl K5) Recombinant Rabbit mAb
Primary antibody: Histone H2B (mono methyl K5) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 14 kDa
Observed MW: 17 kDa
This blot was developed with high sensitivity substrate
WB result of Histone H2B (mono methyl K5) Recombinant Rabbit mAb
Primary antibody: Histone H2B (mono methyl K5) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 14 kDa
Observed MW: 17 kDa
This blot was developed with high sensitivity substrate
Dot Blot
Dot blot result of Histone H2B (mono methyl K5) Recombinant Rabbit mAb
Lane 1: Histone H2B (mono methyl K5) peptide
Lane 2: Histone H2B unmodified peptide
Lane 3: Histone H2B (di methyl K5) peptide
Lane 4: Histone H2B (tri methyl K5) peptide
Primary antibody: Histone H2B (mono methyl K5) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-Histone H2B (mono methyl K5) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Histone H2B (mono methyl K5) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Histone H2B (mono methyl K5) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-Histone H2B (mono methyl K5) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- Histone H2B (mono methyl K5) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- Histone H2B (mono methyl K5) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
