WB result of Histone H2B (Crotonyl K11) Recombinant Rabbit mAb
Primary antibody: Histone H2B (Crotonyl K11) Recombinant Rabbit mAb at 1/500 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Lane 3: untreated HeLa whole cell lysate 20 µg
Lane 4: HeLa treated with 30 mM Sodium Butyrate for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
This blot was developed with high sensitivity substrate
Histone H2B (Crotonyl K11) Recombinant Rabbit mAb (S-R420)
Histone H2B (Crotonyl K11) Recombinant Rabbit mAb (S-R420)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Histone H2B (Crotonyl K11) |
| Synonyms | H2BK11Cr |
| Location | Nucleus |
| Accession | P33778 |
| Clone Number | S-R420 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500 | null |
| ICC | 1:500 | null |
Background
Histone H2B (Crotonyl K11) is a specific form of post-translational modification where the histone H2B protein at lysine 11 is crotonylated, meaning a crotonyl group is added to this amino acid residue. This modification is part of the broader class of histone lysine crotonylation (Kcr), which has emerged as an important epigenetic mark with distinct functions from histone acetylation. Crotonylation involves the addition of an acyl group from an acyl-CoA donor to the ε-amino group of the lysine side chain, which is similar to other acylation modifications but differs in hydrocarbon chain length, hydrophobicity, or charge 。 This modification has been identified in various histone and non-histone proteins, where it influences RNA processing, nucleic acid metabolism, and gene expression.
Picture
Picture
Western Blot
Immunocytochemistry
ICC analysis of HeLa cells treated with TSA (500ng/ml,4h) (top panel) and HeLa cells untreated with TSA (500ng/ml, 4h) (below panel). Anti-Histone H2B (Crotonyl K11) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
