WB result of His tag Recombinant Rabbit mAb (HRP Conjugate)
Primary antibody: His tag Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 1: 293T transfected with His-tagged fusion protein whole cell lysate 4 µg
Predicted MW: 17 kDa
Observed MW: 17 kDa
His tag Recombinant Rabbit mAb (HRP Conjugate) (S-1398-151)
His tag Recombinant Rabbit mAb (HRP Conjugate) (S-1398-151)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | His tag |
| Immunogen | Synthetic Peptide |
| Clone Number | S-1398-151 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Species Independent |
| Positive Sample | 293T transfected with His-tagged fusion protein |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | HRP |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| WB | 1:5000-1:10000 | Species Independent |
Background
A polyhistidine-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. The total number of histidine residues may vary in the tag from as low as two, to as high as 10 or more His residues. This antibody recognizes His-tags placed at N-terminal and C-terminal of fusion proteins.
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Picture
Western Blot
WB result of His tag Recombinant Rabbit mAb (HRP Conjugate)
Primary antibody: His tag Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 1: Angiopoietin-1(277-498) His Tag Protein, Mouse 20 ng
Lane 2: Angiopoietin-2(275-496) His Tag Protein, Human 20 ng
Predicted MW: 28-33 kDa
Observed MW: 30 kDa
