WB result of Heme Oxygenase 1 Recombinant Rabbit mAb
Primary antibody: Heme Oxygenase 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 50 µM arsenite for 8 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 30 kDa
Heme Oxygenase 1 Recombinant Rabbit mAb (S-2614-1)
Heme Oxygenase 1 Recombinant Rabbit mAb (S-2614-1)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Heme Oxygenase 1 |
| Synonyms | Heme oxygenase 1; HO-1Cleaved into 1 chainsHeme oxygenase 1 soluble form; HO; HO1; HMOX1 |
| Immunogen | Recombinant Protein |
| Location | Endoplasmic reticulum |
| Accession | P09601 |
| Clone Number | S-2614-1 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms |
| Positive Sample | A549, HepG2, HT-1080, HeLa, NIH/3T3 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms |
Background
Heme Oxygenase-1 (HO-1, HSP32) is a 32 kDa stress-responsive enzyme anchored to the smooth endoplasmic reticulum that cleaves heme at the α-meso carbon to release equimolar biliverdin (rapidly reduced to bilirubin), carbon monoxide (CO) and ferrous iron, thereby not only eliminating the pro-oxidant heme but also generating potent antioxidants and the signaling gas CO; transcription of the HMOX1 gene is potently induced by myriad stressors—including heme itself, ROS, heavy metals, cytokines, hypoxia, nitric oxide, and natural electrophiles—via Nrf2/ARE, Bach1, HIF-1, AP-1 and NF-κB pathways, giving HO-1 a central cytoprotective role against oxidative and inflammatory injury in cardiovascular, neuronal, renal and hepatic tissues, modulating mitochondrial biogenesis, autophagy and immune tolerance, while its products bilirubin and CO respectively scavenge radicals and activate cGMP/PKG signaling to vasodilate, inhibit platelet aggregation and suppress apoptosis, making HO-1 a therapeutic target in sepsis, ischemia–reperfusion, transplant rejection, metabolic disorders and neurodegeneration.
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Picture
Western Blot
WB result of Heme Oxygenase 1 Recombinant Rabbit mAb
Primary antibody: Heme Oxygenase 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HT-1080 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 30 kDa
WB result of Heme Oxygenase 1 Recombinant Rabbit mAb
Primary antibody: Heme Oxygenase 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 50 µM arsenite for 8 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 30 kDa
