WB result of GSPT1 Recombinant Rabbit mAb
Primary antibody: GSPT1 Recombinant Rabbit mAb at 1/3000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: 293T whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 85 kDa
GSPT1 Recombinant Rabbit mAb (S-1914-49)
GSPT1 Recombinant Rabbit mAb (S-1914-49)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | GSPT1 |
| Synonyms | Eukaryotic peptide chain release factor GTP-binding subunit ERF3A; Eukaryotic peptide chain release factor subunit 3a; eRF3a; G1 to S phase transition protein 1 homolog |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P15170 |
| Clone Number | S-1914-49 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, HepG2, 293T, NIH/3T3, Neuro-2a, PC-12, C6 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:3000 | Hu, Ms, Rt |
| IHC-P | 1:200 | Hu, Ms, Rt |
Background
GSPT1, also known as eukaryotic release factor 3a (eRF3a), is a GTP-binding protein that plays a crucial role in both translation termination and cell cycle regulation. It functions by binding with eRF1 to dissociate proteins from the ribosome after translation, thus regulating cell cycle progression from the G1 to S phase. GSPT1 is overexpressed in various cancers, including gastric, colorectal, lung, and breast cancers, making it a promising target for cancer therapy. Additionally, GSPT1 is involved in cytoskeleton organization and apoptosis. Its degradation through molecular glue degraders, such as CC-885, has been shown to exert strong antitumor activity.
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Western Blot
WB result of GSPT1 Recombinant Rabbit mAb
Primary antibody: GSPT1 Recombinant Rabbit mAb at 1/3000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: Neuro-2a whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 85 kDa
WB result of GSPT1 Recombinant Rabbit mAb
Primary antibody: GSPT1 Recombinant Rabbit mAb at 1/3000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 85 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-GSPT1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-GSPT1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-GSPT1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-GSPT1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-GSPT1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-GSPT1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
