WB result of Granzyme K Recombinant Rabbit mAb
Primary antibody: Granzyme K Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: SH-SY5Y whole cell lysate 20 µg
Lane 3: HuT-78 whole cell lysate 20 µg
Lane 4: NK-92 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate; SH-SY5Y whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 29 kDa
Granzyme K Recombinant Rabbit mAb (S-2665-22)
Granzyme K Recombinant Rabbit mAb (S-2665-22)
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$100 USD
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Granzyme K |
| Synonyms | Fragmentin-3; Granzyme-3; NK-tryptase-2 (NK-Tryp-2); TRYP2; GZMK |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | P49863 |
| Clone Number | S-2665-22 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Positive Sample | HuT-78, NK-92 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
Background
Granzyme K is a serine protease stored in the cytotoxic granules of CD8⁺ T cells and NK cells that, upon perforin-mediated entry into target cells, cleaves the nucleosome-assembly factor SET and the DNA damage-response factor Ku70 to trigger caspase-independent nuclear condensation, mitochondrial perturbation, and rapid cell death, thereby amplifying antiviral and antitumor immunity.
Picture
Picture
Western Blot
