WB result of Granzyme A Rabbit pAb
Primary antibody: Granzyme A Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: NK-92 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 29 kDa
Observed MW: 22~29 kDa
Granzyme A Rabbit Polyclonal Antibody
Granzyme A Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Granzyme A |
| Synonyms | CTL tryptase, Cytotoxic T-lymphocyte proteinase 1, Fragmentin-1, Granzyme-1, Hanukkah factor (H factor; HF); CTLA3; HFSP; GZMA |
| Immunogen | Recombinant Protein |
| Location | Secreted |
| Accession | P12544 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | NK-92 |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:500 | Hu |
Background
Granzyme A (GzmA) is a serine protease predominantly found in the granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It plays a critical role in immune defense by inducing cell death, both apoptotic and non-apoptotic, in target cells such as virus-infected or cancerous cells. Granzyme A is known to specifically target and cleave a range of important human proteins, including histones, nuclear envelope proteins, and key DNA repair proteins like Ku70 and PARP-1. Additionally, it can activate interleukin-1, contributing to pro-inflammatory activities. Recent research has highlighted Granzyme A's ability to trigger a novel programmed cell death pathway, pyroptosis, by cleaving Gasdermin B (GSDMB), which leads to the release of its N-terminal pore-forming fragment and subsequent cell death. This mechanism is particularly relevant in the context of anti-tumor immunity, as it suggests a new way in which cytotoxic lymphocytes can eliminate target cells. The discovery of Granzyme A's role in pyroptosis has provided new insights into the mechanisms of cell death induced by the immune system and offers potential therapeutic targets for enhancing anti-tumor immunity.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human spleen. Anti-Granzyme A antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human NK/T-cell lymphoma. Anti-Granzyme A antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Granzyme A antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
