IHC shows positive staining in paraffin-embedded human epididymis. Anti-GPR64 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
GPR64 Recombinant Rabbit mAb (S-1919-10)
GPR64 Recombinant Rabbit mAb (S-1919-10)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | GPR64 |
| Synonyms | Adhesion G-protein coupled receptor G2; G-protein coupled receptor 64; Human epididymis-specific protein 6 (He6); ADGRG2 |
| Immunogen | Synthetic Peptide |
| Location | Apical cell membrane |
| Accession | Q8IZP9 |
| Clone Number | S-1919-10 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P, IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:500 | Hu |
| IF | 1:500 | Hu |
Background
GPR64, also known as Adhesion G protein-coupled receptor G2 (ADGRG2) or Human Epididymis-specific protein 6 (HE6), is a member of the adhesion G protein-coupled receptor (aGPCR) family and is primarily recognized for its role in male fertility. It is predominantly expressed in the epididymis and parathyroid gland, where it helps maintain ion/pH homeostasis and is involved in sperm maturation and fluid reabsorption. Recent studies have also identified GPR64 in other tissues, such as gastrointestinal tuft cells, where it may play a role in chemosensory functions. Additionally, GPR64 has been implicated in various cancers, including endometrial cancer and Ewing sarcoma, where it may influence tumor growth and metastasis. Despite its diverse functions, the exact mechanisms of GPR64 signaling remain under investigation, with ongoing research aiming to optimize peptide ligands for better receptor characterization.
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Immunohistochemistry
Negative control: IHC shows negative staining in paraffin-embedded human testis. Anti-GPR64 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded Human epididymis. Anti-GPR64 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
