WB result of GORASP2 Recombinant Rabbit mAb
Primary antibody: GORASP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A375 whole cell lysate 20 µg
Lane 2: U-87 MG whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Lane 5: K562 whole cell lysate 20 µg
Low expression control: A375 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 56 kDa
GORASP2 Recombinant Rabbit mAb (S-2526-47)
GORASP2 Recombinant Rabbit mAb (S-2526-47)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | GORASP2 |
| Synonyms | Golgi reassembly-stacking protein 2; GRS2; Golgi phosphoprotein 6 (GOLPH6); Golgi reassembly-stacking protein of 55 kDa (GRASP55); p59; GOLPH6 |
| Immunogen | Synthetic Peptide |
| Location | Endoplasmic reticulum |
| Accession | Q9H8Y8 |
| Clone Number | S-2526-47 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | U-87 MG, HeLa, HepG2, K562, mouse testis, rat testis |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:1000-1:2000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
GORASP2 (also called GRASP55) is a peripheral membrane protein encoded by the GORASP2 gene, located on the medial-trans face of the Golgi apparatus, where it acts as a "molecular glue" by forming trans-oligomers through its conserved N-terminal GRASP domain to stack adjacent cisternae into the characteristic Golgi ribbon . In normal growth conditions it is O-GlcNAcylated, but upon glucose or energy starvation it is rapidly de-O-GlcNAcylated and a subpopulation relocates to the autophagosome–lysosome interface, where it tethers LC3-II on autophagosomes to LAMP2 on lysosomes, thereby facilitating autophagosome–lysosome fusion and promoting autophagosome maturation as an unconventional energy sensor.
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Western Blot
WB result of GORASP2 Recombinant Rabbit mAb
Primary antibody: GORASP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 56 kDa
WB result of GORASP2 Recombinant Rabbit mAb
Primary antibody: GORASP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 56 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-GORASP2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate. Anti-GORASP2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-GORASP2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-GORASP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-GORASP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-GORASP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-GORASP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-GORASP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- GORASP2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
